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two color real time pcr detection system  (Bio-Rad)


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    Structured Review

    Bio-Rad two color real time pcr detection system
    Two Color Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 8618 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+color+real+time+pcr+detection+system/Sso+Advanced+Universal+Mix+Con+Sybr+Green/pm41986344-434-9-14
    Average 99 stars, based on 8618 article reviews
    two color real time pcr detection system - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Postoperative Electroacupuncture Boosts Cognitive Function Recovery after Laparotomy in Mice
    Article Snippet: Subsequently, 0.5 μg of RNA was used for reverse transcription with the PrimeScript TM Master Mix Kit (Perfect Real Time) (Takara, Japan), and cDNA samples were diluted tenfold in DEPC-treated water. .. Five microliters of diluted cDNA were amplified in triplicate using the TB Premix Ex TaqTM II kit (Takara, Shiga, Japan) and the CFX96 Touch Two-Color Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). ..

    Article Title: IL-1β and TNF-α play an important role in modulating the risk of periodontitis and Alzheimer’s disease
    Article Snippet: RNA samples (0.5 μg) were then subsequently used for reverse transcription using PrimeScriptTM Master Mix Kit (Perfect Real Time) (Takara, Japan), and the cDNA samples were further diluted 10 times in DEPC-treated water. .. Five μl of diluted cDNA samples were amplified in triplicate using TB Premix Ex TaqTM II kit (Takara, Japan) and the CFX96 Touch two-color Real-Time PCR detection System (Bio-Rad, Hercules, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Postoperative Electroacupuncture Boosts Cognitive Function Recovery after Laparotomy in Mice
    Article Snippet: Subsequently, 0.5 μg of RNA was used for reverse transcription with the PrimeScript TM Master Mix Kit (Perfect Real Time) (Takara, Japan), and cDNA samples were diluted tenfold in DEPC-treated water. .. Five microliters of diluted cDNA were amplified in triplicate using the TB Premix Ex TaqTM II kit (Takara, Shiga, Japan) and the CFX96 Touch Two-Color Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer.
    Article Snippet: For quantitative reverse transcription-polymerase chain reaction (qRTPCR), total RNA was extracted using the TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific, Cat# 12183555) and reverse-transcribed into cDNA. .. Real-time PCR was performed with gene-specific primers on the two-color real-time PCR detection system (Bio-Rad) using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Cat# 1725272) to measure relative expression levels. .. The sequences of the primers (IDT) used were: SOX10 FW: 5′-CCTGAGGTGGGCAAGGAAC-3′; SOX10 REV: 5′-AGGACCCTATTATGGCCACTCG-3′; NFKBIE FW: 5′- CCCGGTTGGTCCAGATGTAC-3′, NFKBIE REV: 5′-GGGATCCCAAGAGCTTGGTG-3′.

    Article Title: Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer
    Article Snippet: For quantitative reverse transcription-polymerase chain reaction (qRT-PCR), total RNA was extracted using the TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific, Cat# 12183555) and reverse-transcribed into cDNA. .. Real-time PCR was performed with gene-specific primers on the two-color real-time PCR detection system (Bio-Rad) using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Cat# 1725272) to measure relative expression levels. .. The sequences of the primers (IDT) used were: SOX10 FW: 5′-CCTGAGGTGGGCAAGGAAC-3′; SOX10 REV: 5′-AGGACCCTATTATGGCCACTCG-3′; NFKBIE FW: 5′-CCCGGTTGGTCCAGATGTAC-3′, NFKBIE REV: 5′-GGGATCCCAAGAGCTTGGTG-3′.

    Article Title: Enhancement of gold-curcumin nanoparticle mediated radiation response for improved therapy in cervical cancer: a computational approach and predictive pathway analysis
    Article Snippet: .. After that total RNA was isolated by TRIzol® reagent (Invitrogen-life technologies) according to the manufacturer’s protocol. cDNA was synthesized by using 1 μg of total RNA, then qRT-PCR was performed with SYBRTMGreen qPCR Master Mix (Thermo Scientific) on the MyiQTM Two-color Real-time PCR detection system (BioRad). .. The primer sequences were designed in our laboratory on Primer Premier software and purchased from Eurofins Scientifics.

    Article Title: Circulating exosome-derived miR-191-5p is a novel therapeutic biomarker for radiotherapy in esophageal squamous cell carcinoma patients.
    Article Snippet: .. The reaction mixture was incubated at 16 °C for 30 min, 42 °C for 60 min, and 85 °C for 5 min. After cDNA conversion, RT-PCR was performed in triplicate using the MyiQ2 Two-Color Real-Time PCR Detection System (BIO-RAD, Hercules, CA). ..

    Article Title: Conjoint Analysis of Genome-Wide lncRNA and mRNA Expression during the Salicylic Acid Response in Populus × euramericana
    Article Snippet: .. The qRT-PCR reaction was performed with the MiniOpticon Two-Color Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA), using SuperReal PreMix Plus (Tiangen, Beijing, China). ..

    Article Title: IL-1β and TNF-α play an important role in modulating the risk of periodontitis and Alzheimer’s disease
    Article Snippet: RNA samples (0.5 μg) were then subsequently used for reverse transcription using PrimeScriptTM Master Mix Kit (Perfect Real Time) (Takara, Japan), and the cDNA samples were further diluted 10 times in DEPC-treated water. .. Five μl of diluted cDNA samples were amplified in triplicate using TB Premix Ex TaqTM II kit (Takara, Japan) and the CFX96 Touch two-color Real-Time PCR detection System (Bio-Rad, Hercules, USA). ..

    Article Title: IL-1β and TNF-α play an important role in modulating the risk of periodontitis and Alzheimer’s disease
    Article Snippet: RNA samples (0.5 mg) and then subsequently used for reverse transcription using PrimeScriptTM Master Mix Kit (Perfect Real Time) (Takara, Japan) and the cDNA samples were further diluted 10 times in DEPCtreated water. .. Real-Time PCR Five μl of diluted cDNA samples were ampli ed in triplicate using TB Premix Ex TaqTM II kit (Takara, Japan) and the CFX96 Touch two-color Real-Time PCR detection System (Bio-Rad, Hercules, USA). ..

    SYBR Green Assay:

    Article Title: Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer.
    Article Snippet: For quantitative reverse transcription-polymerase chain reaction (qRTPCR), total RNA was extracted using the TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific, Cat# 12183555) and reverse-transcribed into cDNA. .. Real-time PCR was performed with gene-specific primers on the two-color real-time PCR detection system (Bio-Rad) using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Cat# 1725272) to measure relative expression levels. .. The sequences of the primers (IDT) used were: SOX10 FW: 5′-CCTGAGGTGGGCAAGGAAC-3′; SOX10 REV: 5′-AGGACCCTATTATGGCCACTCG-3′; NFKBIE FW: 5′- CCCGGTTGGTCCAGATGTAC-3′, NFKBIE REV: 5′-GGGATCCCAAGAGCTTGGTG-3′.

    Article Title: Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer
    Article Snippet: For quantitative reverse transcription-polymerase chain reaction (qRT-PCR), total RNA was extracted using the TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific, Cat# 12183555) and reverse-transcribed into cDNA. .. Real-time PCR was performed with gene-specific primers on the two-color real-time PCR detection system (Bio-Rad) using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Cat# 1725272) to measure relative expression levels. .. The sequences of the primers (IDT) used were: SOX10 FW: 5′-CCTGAGGTGGGCAAGGAAC-3′; SOX10 REV: 5′-AGGACCCTATTATGGCCACTCG-3′; NFKBIE FW: 5′-CCCGGTTGGTCCAGATGTAC-3′, NFKBIE REV: 5′-GGGATCCCAAGAGCTTGGTG-3′.

    Expressing:

    Article Title: Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer.
    Article Snippet: For quantitative reverse transcription-polymerase chain reaction (qRTPCR), total RNA was extracted using the TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific, Cat# 12183555) and reverse-transcribed into cDNA. .. Real-time PCR was performed with gene-specific primers on the two-color real-time PCR detection system (Bio-Rad) using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Cat# 1725272) to measure relative expression levels. .. The sequences of the primers (IDT) used were: SOX10 FW: 5′-CCTGAGGTGGGCAAGGAAC-3′; SOX10 REV: 5′-AGGACCCTATTATGGCCACTCG-3′; NFKBIE FW: 5′- CCCGGTTGGTCCAGATGTAC-3′, NFKBIE REV: 5′-GGGATCCCAAGAGCTTGGTG-3′.

    Article Title: Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer
    Article Snippet: For quantitative reverse transcription-polymerase chain reaction (qRT-PCR), total RNA was extracted using the TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific, Cat# 12183555) and reverse-transcribed into cDNA. .. Real-time PCR was performed with gene-specific primers on the two-color real-time PCR detection system (Bio-Rad) using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Cat# 1725272) to measure relative expression levels. .. The sequences of the primers (IDT) used were: SOX10 FW: 5′-CCTGAGGTGGGCAAGGAAC-3′; SOX10 REV: 5′-AGGACCCTATTATGGCCACTCG-3′; NFKBIE FW: 5′-CCCGGTTGGTCCAGATGTAC-3′, NFKBIE REV: 5′-GGGATCCCAAGAGCTTGGTG-3′.

    Isolation:

    Article Title: Enhancement of gold-curcumin nanoparticle mediated radiation response for improved therapy in cervical cancer: a computational approach and predictive pathway analysis
    Article Snippet: .. After that total RNA was isolated by TRIzol® reagent (Invitrogen-life technologies) according to the manufacturer’s protocol. cDNA was synthesized by using 1 μg of total RNA, then qRT-PCR was performed with SYBRTMGreen qPCR Master Mix (Thermo Scientific) on the MyiQTM Two-color Real-time PCR detection system (BioRad). .. The primer sequences were designed in our laboratory on Primer Premier software and purchased from Eurofins Scientifics.

    Synthesized:

    Article Title: Enhancement of gold-curcumin nanoparticle mediated radiation response for improved therapy in cervical cancer: a computational approach and predictive pathway analysis
    Article Snippet: .. After that total RNA was isolated by TRIzol® reagent (Invitrogen-life technologies) according to the manufacturer’s protocol. cDNA was synthesized by using 1 μg of total RNA, then qRT-PCR was performed with SYBRTMGreen qPCR Master Mix (Thermo Scientific) on the MyiQTM Two-color Real-time PCR detection system (BioRad). .. The primer sequences were designed in our laboratory on Primer Premier software and purchased from Eurofins Scientifics.

    Quantitative RT-PCR:

    Article Title: Enhancement of gold-curcumin nanoparticle mediated radiation response for improved therapy in cervical cancer: a computational approach and predictive pathway analysis
    Article Snippet: .. After that total RNA was isolated by TRIzol® reagent (Invitrogen-life technologies) according to the manufacturer’s protocol. cDNA was synthesized by using 1 μg of total RNA, then qRT-PCR was performed with SYBRTMGreen qPCR Master Mix (Thermo Scientific) on the MyiQTM Two-color Real-time PCR detection system (BioRad). .. The primer sequences were designed in our laboratory on Primer Premier software and purchased from Eurofins Scientifics.

    Article Title: Conjoint Analysis of Genome-Wide lncRNA and mRNA Expression during the Salicylic Acid Response in Populus × euramericana
    Article Snippet: .. The qRT-PCR reaction was performed with the MiniOpticon Two-Color Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA), using SuperReal PreMix Plus (Tiangen, Beijing, China). ..

    Incubation:

    Article Title: Circulating exosome-derived miR-191-5p is a novel therapeutic biomarker for radiotherapy in esophageal squamous cell carcinoma patients.
    Article Snippet: .. The reaction mixture was incubated at 16 °C for 30 min, 42 °C for 60 min, and 85 °C for 5 min. After cDNA conversion, RT-PCR was performed in triplicate using the MyiQ2 Two-Color Real-Time PCR Detection System (BIO-RAD, Hercules, CA). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Circulating exosome-derived miR-191-5p is a novel therapeutic biomarker for radiotherapy in esophageal squamous cell carcinoma patients.
    Article Snippet: .. The reaction mixture was incubated at 16 °C for 30 min, 42 °C for 60 min, and 85 °C for 5 min. After cDNA conversion, RT-PCR was performed in triplicate using the MyiQ2 Two-Color Real-Time PCR Detection System (BIO-RAD, Hercules, CA). ..



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